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recombinant βngf  (Alomone Labs)
93
Alomone Labs recombinant βngf
( A ) Carboxy-terminal sequences of NGF, highlighting prospective CendR motifs (R/KxxR/K). Amino acids numbered according to the mature <t>βNGF</t> sequence (1-120 equivalent to proNGF 122-241). h, Homo sapiens; r, Rattus norvegicus; m, Mus musculus . ( B ) Prospective binding site between human βNGF and NRP1 modelled using protein docking PIPER software. ( C ) Interaction between purified His-tagged human NRP1 (residues 22-644) and unlabeled human beta-NGF using MST. Data from 4 independent experiments. Mean±SEM. ( D-E ) Bioluminescence resonance energy transfer (BRET) to measure proximity (<10 nm) at full-length NRP1 in living HEK293T cells at 37°C. Supernatant was collected from cells secreting growth factor (VEGF 165 a or NGF) tagged with HiBiT, a small portion of nanoluciferase with high affinity. When reconstituted with <t>recombinant</t> LgBiT and luciferase substrate, this acts as a bioluminescent donor for SnapTag-NRP1 labeled with SNAPTag-Alexa Fluor® 488 (AF488). BRET was compared to negative control (HiBiT/LgBiT only lacking AF488). Cells were pre-incubated with vehicle or 10 nM unlabeled VEGF 165 a (30 min), followed by luminescent growth factor (15 min, 37°C). BRET with HiBiT-VEGF 165 a was measured between HiBiT-VEGF 165 a and either SnapTag-NRP1 (WT) or the known VEGF 165 a binding-dead mutant (Y297A), as well as HiBiT-tagged NGF ( E ). Data from 4 independent experiments with triplicate wells. F. 1-way ANOVA, Šídák’s multiple comparisons. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Recombinant βngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human βngf  (R&D Systems)
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R&D Systems human βngf
( A ) Carboxy-terminal sequences of NGF, highlighting prospective CendR motifs (R/KxxR/K). Amino acids numbered according to the mature <t>βNGF</t> sequence (1-120 equivalent to proNGF 122-241). h, Homo sapiens; r, Rattus norvegicus; m, Mus musculus . ( B ) Prospective binding site between human βNGF and NRP1 modelled using protein docking PIPER software. ( C ) Interaction between purified His-tagged human NRP1 (residues 22-644) and unlabeled human beta-NGF using MST. Data from 4 independent experiments. Mean±SEM. ( D-E ) Bioluminescence resonance energy transfer (BRET) to measure proximity (<10 nm) at full-length NRP1 in living HEK293T cells at 37°C. Supernatant was collected from cells secreting growth factor (VEGF 165 a or NGF) tagged with HiBiT, a small portion of nanoluciferase with high affinity. When reconstituted with <t>recombinant</t> LgBiT and luciferase substrate, this acts as a bioluminescent donor for SnapTag-NRP1 labeled with SNAPTag-Alexa Fluor® 488 (AF488). BRET was compared to negative control (HiBiT/LgBiT only lacking AF488). Cells were pre-incubated with vehicle or 10 nM unlabeled VEGF 165 a (30 min), followed by luminescent growth factor (15 min, 37°C). BRET with HiBiT-VEGF 165 a was measured between HiBiT-VEGF 165 a and either SnapTag-NRP1 (WT) or the known VEGF 165 a binding-dead mutant (Y297A), as well as HiBiT-tagged NGF ( E ). Data from 4 independent experiments with triplicate wells. F. 1-way ANOVA, Šídák’s multiple comparisons. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Human βngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+%CE%B2ngf/pm41397289-129-58-60?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
human βngf - by Bioz Stars, 2026-07
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recombinant βngf  (R&D Systems)
92
R&D Systems recombinant βngf
( A ) Carboxy-terminal sequences of NGF, highlighting prospective CendR motifs (R/KxxR/K). Amino acids numbered according to the mature <t>βNGF</t> sequence (1-120 equivalent to proNGF 122-241). h, Homo sapiens; r, Rattus norvegicus; m, Mus musculus . ( B ) Prospective binding site between human βNGF and NRP1 modelled using protein docking PIPER software. ( C ) Interaction between purified His-tagged human NRP1 (residues 22-644) and unlabeled human beta-NGF using MST. Data from 4 independent experiments. Mean±SEM. ( D-E ) Bioluminescence resonance energy transfer (BRET) to measure proximity (<10 nm) at full-length NRP1 in living HEK293T cells at 37°C. Supernatant was collected from cells secreting growth factor (VEGF 165 a or NGF) tagged with HiBiT, a small portion of nanoluciferase with high affinity. When reconstituted with <t>recombinant</t> LgBiT and luciferase substrate, this acts as a bioluminescent donor for SnapTag-NRP1 labeled with SNAPTag-Alexa Fluor® 488 (AF488). BRET was compared to negative control (HiBiT/LgBiT only lacking AF488). Cells were pre-incubated with vehicle or 10 nM unlabeled VEGF 165 a (30 min), followed by luminescent growth factor (15 min, 37°C). BRET with HiBiT-VEGF 165 a was measured between HiBiT-VEGF 165 a and either SnapTag-NRP1 (WT) or the known VEGF 165 a binding-dead mutant (Y297A), as well as HiBiT-tagged NGF ( E ). Data from 4 independent experiments with triplicate wells. F. 1-way ANOVA, Šídák’s multiple comparisons. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Recombinant βngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+%CE%B2ngf/pmc11827847__jci___135___183873___s034-12-0-2?v=R%26D+Systems
Average 92 stars, based on 1 article reviews
recombinant βngf - by Bioz Stars, 2026-07
92/100 stars
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βngf grade  (Alomone Labs)
93
Alomone Labs βngf grade
( A ) Carboxy-terminal sequences of NGF, highlighting prospective CendR motifs (R/KxxR/K). Amino acids numbered according to the mature <t>βNGF</t> sequence (1-120 equivalent to proNGF 122-241). h, Homo sapiens; r, Rattus norvegicus; m, Mus musculus . ( B ) Prospective binding site between human βNGF and NRP1 modelled using protein docking PIPER software. ( C ) Interaction between purified His-tagged human NRP1 (residues 22-644) and unlabeled human beta-NGF using MST. Data from 4 independent experiments. Mean±SEM. ( D-E ) Bioluminescence resonance energy transfer (BRET) to measure proximity (<10 nm) at full-length NRP1 in living HEK293T cells at 37°C. Supernatant was collected from cells secreting growth factor (VEGF 165 a or NGF) tagged with HiBiT, a small portion of nanoluciferase with high affinity. When reconstituted with <t>recombinant</t> LgBiT and luciferase substrate, this acts as a bioluminescent donor for SnapTag-NRP1 labeled with SNAPTag-Alexa Fluor® 488 (AF488). BRET was compared to negative control (HiBiT/LgBiT only lacking AF488). Cells were pre-incubated with vehicle or 10 nM unlabeled VEGF 165 a (30 min), followed by luminescent growth factor (15 min, 37°C). BRET with HiBiT-VEGF 165 a was measured between HiBiT-VEGF 165 a and either SnapTag-NRP1 (WT) or the known VEGF 165 a binding-dead mutant (Y297A), as well as HiBiT-tagged NGF ( E ). Data from 4 independent experiments with triplicate wells. F. 1-way ANOVA, Šídák’s multiple comparisons. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
βngf Grade, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+%CE%B2ngf/pmc02763125-39-5-13?v=Alomone+Labs
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βngf grade - by Bioz Stars, 2026-07
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human recombinant βngf  (PeproTech)
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PeproTech human recombinant βngf
( A ) Carboxy-terminal sequences of NGF, highlighting prospective CendR motifs (R/KxxR/K). Amino acids numbered according to the mature <t>βNGF</t> sequence (1-120 equivalent to proNGF 122-241). h, Homo sapiens; r, Rattus norvegicus; m, Mus musculus . ( B ) Prospective binding site between human βNGF and NRP1 modelled using protein docking PIPER software. ( C ) Interaction between purified His-tagged human NRP1 (residues 22-644) and unlabeled human beta-NGF using MST. Data from 4 independent experiments. Mean±SEM. ( D-E ) Bioluminescence resonance energy transfer (BRET) to measure proximity (<10 nm) at full-length NRP1 in living HEK293T cells at 37°C. Supernatant was collected from cells secreting growth factor (VEGF 165 a or NGF) tagged with HiBiT, a small portion of nanoluciferase with high affinity. When reconstituted with <t>recombinant</t> LgBiT and luciferase substrate, this acts as a bioluminescent donor for SnapTag-NRP1 labeled with SNAPTag-Alexa Fluor® 488 (AF488). BRET was compared to negative control (HiBiT/LgBiT only lacking AF488). Cells were pre-incubated with vehicle or 10 nM unlabeled VEGF 165 a (30 min), followed by luminescent growth factor (15 min, 37°C). BRET with HiBiT-VEGF 165 a was measured between HiBiT-VEGF 165 a and either SnapTag-NRP1 (WT) or the known VEGF 165 a binding-dead mutant (Y297A), as well as HiBiT-tagged NGF ( E ). Data from 4 independent experiments with triplicate wells. F. 1-way ANOVA, Šídák’s multiple comparisons. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Human Recombinant βngf, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+%CE%B2ngf/pmc07176653-187-24-33?v=PeproTech
Average 90 stars, based on 1 article reviews
human recombinant βngf - by Bioz Stars, 2026-07
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mouse βngf  (Alomone Labs)
93
Alomone Labs mouse βngf
( A ) Carboxy-terminal sequences of NGF, highlighting prospective CendR motifs (R/KxxR/K). Amino acids numbered according to the mature <t>βNGF</t> sequence (1-120 equivalent to proNGF 122-241). h, Homo sapiens; r, Rattus norvegicus; m, Mus musculus . ( B ) Prospective binding site between human βNGF and NRP1 modelled using protein docking PIPER software. ( C ) Interaction between purified His-tagged human NRP1 (residues 22-644) and unlabeled human beta-NGF using MST. Data from 4 independent experiments. Mean±SEM. ( D-E ) Bioluminescence resonance energy transfer (BRET) to measure proximity (<10 nm) at full-length NRP1 in living HEK293T cells at 37°C. Supernatant was collected from cells secreting growth factor (VEGF 165 a or NGF) tagged with HiBiT, a small portion of nanoluciferase with high affinity. When reconstituted with <t>recombinant</t> LgBiT and luciferase substrate, this acts as a bioluminescent donor for SnapTag-NRP1 labeled with SNAPTag-Alexa Fluor® 488 (AF488). BRET was compared to negative control (HiBiT/LgBiT only lacking AF488). Cells were pre-incubated with vehicle or 10 nM unlabeled VEGF 165 a (30 min), followed by luminescent growth factor (15 min, 37°C). BRET with HiBiT-VEGF 165 a was measured between HiBiT-VEGF 165 a and either SnapTag-NRP1 (WT) or the known VEGF 165 a binding-dead mutant (Y297A), as well as HiBiT-tagged NGF ( E ). Data from 4 independent experiments with triplicate wells. F. 1-way ANOVA, Šídák’s multiple comparisons. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Mouse βngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+%CE%B2ngf/pm30903486-50-1-8?v=Alomone+Labs
Average 93 stars, based on 1 article reviews
mouse βngf - by Bioz Stars, 2026-07
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high grade mouse βngf  (Alomone Labs)
93
Alomone Labs high grade mouse βngf
( A ) Carboxy-terminal sequences of NGF, highlighting prospective CendR motifs (R/KxxR/K). Amino acids numbered according to the mature <t>βNGF</t> sequence (1-120 equivalent to proNGF 122-241). h, Homo sapiens; r, Rattus norvegicus; m, Mus musculus . ( B ) Prospective binding site between human βNGF and NRP1 modelled using protein docking PIPER software. ( C ) Interaction between purified His-tagged human NRP1 (residues 22-644) and unlabeled human beta-NGF using MST. Data from 4 independent experiments. Mean±SEM. ( D-E ) Bioluminescence resonance energy transfer (BRET) to measure proximity (<10 nm) at full-length NRP1 in living HEK293T cells at 37°C. Supernatant was collected from cells secreting growth factor (VEGF 165 a or NGF) tagged with HiBiT, a small portion of nanoluciferase with high affinity. When reconstituted with <t>recombinant</t> LgBiT and luciferase substrate, this acts as a bioluminescent donor for SnapTag-NRP1 labeled with SNAPTag-Alexa Fluor® 488 (AF488). BRET was compared to negative control (HiBiT/LgBiT only lacking AF488). Cells were pre-incubated with vehicle or 10 nM unlabeled VEGF 165 a (30 min), followed by luminescent growth factor (15 min, 37°C). BRET with HiBiT-VEGF 165 a was measured between HiBiT-VEGF 165 a and either SnapTag-NRP1 (WT) or the known VEGF 165 a binding-dead mutant (Y297A), as well as HiBiT-tagged NGF ( E ). Data from 4 independent experiments with triplicate wells. F. 1-way ANOVA, Šídák’s multiple comparisons. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
High Grade Mouse βngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+%CE%B2ngf/pm28063984-45-0-8?v=Alomone+Labs
Average 93 stars, based on 1 article reviews
high grade mouse βngf - by Bioz Stars, 2026-07
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Image Search Results


( A ) Carboxy-terminal sequences of NGF, highlighting prospective CendR motifs (R/KxxR/K). Amino acids numbered according to the mature βNGF sequence (1-120 equivalent to proNGF 122-241). h, Homo sapiens; r, Rattus norvegicus; m, Mus musculus . ( B ) Prospective binding site between human βNGF and NRP1 modelled using protein docking PIPER software. ( C ) Interaction between purified His-tagged human NRP1 (residues 22-644) and unlabeled human beta-NGF using MST. Data from 4 independent experiments. Mean±SEM. ( D-E ) Bioluminescence resonance energy transfer (BRET) to measure proximity (<10 nm) at full-length NRP1 in living HEK293T cells at 37°C. Supernatant was collected from cells secreting growth factor (VEGF 165 a or NGF) tagged with HiBiT, a small portion of nanoluciferase with high affinity. When reconstituted with recombinant LgBiT and luciferase substrate, this acts as a bioluminescent donor for SnapTag-NRP1 labeled with SNAPTag-Alexa Fluor® 488 (AF488). BRET was compared to negative control (HiBiT/LgBiT only lacking AF488). Cells were pre-incubated with vehicle or 10 nM unlabeled VEGF 165 a (30 min), followed by luminescent growth factor (15 min, 37°C). BRET with HiBiT-VEGF 165 a was measured between HiBiT-VEGF 165 a and either SnapTag-NRP1 (WT) or the known VEGF 165 a binding-dead mutant (Y297A), as well as HiBiT-tagged NGF ( E ). Data from 4 independent experiments with triplicate wells. F. 1-way ANOVA, Šídák’s multiple comparisons. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Journal: bioRxiv

Article Title: Neuropilin-1 is a co-receptor for NGF and TrkA-evoked pain

doi: 10.1101/2023.12.06.570398

Figure Lengend Snippet: ( A ) Carboxy-terminal sequences of NGF, highlighting prospective CendR motifs (R/KxxR/K). Amino acids numbered according to the mature βNGF sequence (1-120 equivalent to proNGF 122-241). h, Homo sapiens; r, Rattus norvegicus; m, Mus musculus . ( B ) Prospective binding site between human βNGF and NRP1 modelled using protein docking PIPER software. ( C ) Interaction between purified His-tagged human NRP1 (residues 22-644) and unlabeled human beta-NGF using MST. Data from 4 independent experiments. Mean±SEM. ( D-E ) Bioluminescence resonance energy transfer (BRET) to measure proximity (<10 nm) at full-length NRP1 in living HEK293T cells at 37°C. Supernatant was collected from cells secreting growth factor (VEGF 165 a or NGF) tagged with HiBiT, a small portion of nanoluciferase with high affinity. When reconstituted with recombinant LgBiT and luciferase substrate, this acts as a bioluminescent donor for SnapTag-NRP1 labeled with SNAPTag-Alexa Fluor® 488 (AF488). BRET was compared to negative control (HiBiT/LgBiT only lacking AF488). Cells were pre-incubated with vehicle or 10 nM unlabeled VEGF 165 a (30 min), followed by luminescent growth factor (15 min, 37°C). BRET with HiBiT-VEGF 165 a was measured between HiBiT-VEGF 165 a and either SnapTag-NRP1 (WT) or the known VEGF 165 a binding-dead mutant (Y297A), as well as HiBiT-tagged NGF ( E ). Data from 4 independent experiments with triplicate wells. F. 1-way ANOVA, Šídák’s multiple comparisons. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Article Snippet: Recombinant βNGF (R&D Systems or Alomone Labs) and recombinant NRP1 (R&D Systems) were reconstituted according to manufacturer’s instructions.

Techniques: Sequencing, Binding Assay, Software, Purification, Bioluminescence Resonance Energy Transfer, Recombinant, Luciferase, Labeling, Negative Control, Incubation, Mutagenesis